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Open source and low cost pulsed-field gel electrophoresis [PFGE] equipment

Open source and low cost pulsed-field gel electrophoresis [PFGE] equipment.

Pulsed-field gel electrophoresis [PFGE] is a technique that allows the separation of very large DNA molecules (up to ~10 Mbp / in contrast of up to ~50 Kbp of normal electrophoresis) by a periodic change in the direction of the electric field.

This work considers the design and construction of a open source and low cost PFGE, implementing a Rotating Gel Electrophoresis (RGE). The equipment considers the use of a standard commercially available electrophoresis chamber and the construction of an RGE using 3D printed parts, a servo motor and simple electronics. It incorporates a custom cooling system using a small pump and peltier cooler elements. Run parameters set and monitoring is achieved by bluetooth communication to an smartphone running a custom Android app.

The equipment is capable of the separation of DNA molecules up to ~2 Mbp (10 Mpb is about to be tested), costs USD ~$500 (about 1/50 of the price of commercial equipment, considering the chamber and the power source) and all coding, electronics, 3D parts and documentation is public available on GitLab.

CONTEXTO
TÍTULO
Open source and low cost pulsed-field gel electrophoresis [PFGE] equipment
TIPO DE PROYECTO
CATEGORÍAS
INDUSTRIA
FECHA DE CONSTRUCCIÓN
1 de Octubre de 2019