Hyper drive modificado
Adaptacion de hyperdrive: aditamento usado en modelos de analisis de ondas cerebrales en ratas vivas.
Se recomienda no usar un material sensible a temperaturas mayores a los 60° y con sensibilidad al PMMA dental.
Cualquier duda de su uso revisar el link de referencia con el protocolo.
Chronic recording of large populations of neurons is a valuable technique for studying the function of neuronal circuits in awake behaving rats. Lightweight recording devices carrying a high density array of tetrodes allow for the simultaneous monitoring of the activity of tens to hundreds of individual neurons. Here we describe a protocol for the fabrication of a micro-drive array with twenty one independently movable micro-drives. This device has been used successfully to record from hippocampal and cortical neurons in our lab. We show how to prepare a custom designed, 3-D printed plastic base that will hold the micro-drives. We demonstrate how to construct the individual micro-drives and how to assemble the complete micro-drive array. Further preparation of the drive array for surgical implantation, such as the fabrication of tetrodes, loading of tetrodes into the drive array and gold-plating, is covered in a subsequent video article.